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Antioxidant-Like Properties of Zinc in Activated Endothelial Cells

Departments of Nutrition and Food Science (B.H., P.M.), Neurosurgery (M.T.), and Medicine (C.J.M.), University of Kentucky, Lexington, Kentucky

View larger version (39K): [in a new window]   Fig. 1. Oxidative stress measurement in endothelial cells during zinc deficiency and supplementation and after exposure to linoleic acid (FA) or TNF. Cells were cultured for 8 days in media supplemented with only 1% FBS and subsequently enriched either with zinc (10 µM) for 48 hours and/or treated with FA (90 µM) for 6 hours or TNF (500 U/mL) for 1.5 hours. Values are mean±SEM (n=6). *Significantly different from respective cultures without added zinc.

View larger version (53K): [in a new window]   Fig. 2. Effect of linoleic acid (FA) and added zinc on activation of NF-B. Cells were cultured for 8 days in media supplemented with 1% FBS and appropriate groups enriched with zinc (10 µM) for 48 hours and treatment with FA (90 µM) for 6 hours. Lane 1 = positive control (HeLa cell extract); lane 2 = competitor (unlabeled oligonucleotide); lane 3 = cells cultured in M199 enriched with 1% FBS (control); lane 4 = control + zinc (10 µM); lane 5=control + FA (90 µM); lane 6=control + zinc + FA.

View larger version (34K): [in a new window]   Fig. 3. Effect of linoleic acid (FA) and added zinc on activation of AP-1. Cells were cultured for 8 days in media supplemented with 1% FBS and appropriate groups enriched with zinc (10 µM) for 48 hours and treatment with FA (90 µM) for 6 hours. Lane 1=positive control (HeLa cell extract); lane 2=competitor (unlabeled oligonucleotide); lane 3=cells cultured in M199 enriched with 1% FBS (control); lane 4=control + zinc (10 µM); lane 5=control + FA (90 µM); lane 6=control + zinc + FA.

View larger version (30K): [in a new window]   Fig. 4. IL-6 production in endothelial cells during zinc deficiency and supplementation and after exposure to linoleic acid (FA) or TNF. Cells were culture for 8 days in media supplemented with only 1% FBS and subsequently enriched either with zinc (10 µM) for 48 hours and/or treated with FA or TNF for 12 hours. Values are mean±SEM (n=6). *Significantly different from respective cultures without added zinc.