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Foods as Production and Delivery Vehicles for Human Vaccines

Department of Natural Resources & Environmental Sciences, 310 ERML (S.S.K., S.F.K.), University of Illinois, Urbana, Illinois
Department of Molecular and Integrative Physiology (D.E.B.), University of Illinois, Urbana, Illinois

View larger version (101K): [in a new window]   Fig. 1. Southern blot analysis of tomato lines. Lanes 1–5 correspond to transformed tomato lines carrying the RSV-F transgene and showing a single copy of the transgene; lane 6 corresponds to an untransformed tomato plant (control), and lane 7 corresponds to the plasmid DNA showing the RSV-F antigen.

View larger version (108K): [in a new window]   Fig. 2. Fertile transgenic tomato plants carrying the RSV-F antigen growing in the greenhouse.

View larger version (90K): [in a new window]   Fig. 3. The level of recombinant RSV-F protein in fruit of different tomato plants. Tomato plants were transformed with a construct carrying the RSV-F antigen gene driven by either the CaMV 35S promoter (plasmid pJSS-3) or the E-8 fruit-specific promoter (plasmid pJSS-4). Untransformed tomato plant used as control.

View larger version (115K): [in a new window]   Fig. 4. Localization of the RSV-F antigen in a tomato fruit.