HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Prasad, K. N. Articles by Cole, W. C. Search for Related Content PubMed PubMed Citation Articles by Prasad, K. N. Articles by Cole, W. C.

-Tocopheryl Succinate, the Most Effective Form of Vitamin E for Adjuvant Cancer Treatment: A Review

Center for Vitamins and Cancer Research, Department of Radiology, University of Colorado Health Sciences Center, Denver, Colorado

View larger version (23K): [in a new window]   Fig. 1. Effect of various forms of tocopherol (vitamin E) on the growth of mouse melanoma (B-16) cells in culture. Cells (105) were plated in Lux tissue culture dishes (60 mm). Twenty-four hours after plating, D--tocopherol acid succinate (Sigma; soluble in ethanol), DL--tocopherol free alcohol (Hoffmann-La Roche; soluble in specialized solvent) and Aquasol DL--tocopherol acetate (USV Laboratories; soluble in specialized solvent) at various concentrations were added individually to separate dishes. Growth medium and drug were changed at two days after treatment, and the growth inhibition, based on the amount of protein per dish, in the treated culture was determined at three days after treatment. The average value of the untreated controls was considered 100%, and the growth inhibition of treated cultures was expressed as percentage of untreated controls. The amount of protein per dish in the untreated cultures was 850 ± 67 µg. Each point on the curve represents an average of nine samples; bars, S.D.[7].

View larger version (168K): [in a new window]   Fig. 2. Melanoma cells (105) were plated in Lux tissue culture dishes (60 mm), and D--tocopherol (vitamin E) acid succinate (soluble in ethanol) and sodium succinate plus ethanol were added to separate cultures 24 hours after plating. Drugs and medium were changed at two and three days after treatment. Photomicrographs were taken four days after treatment. Control culture contained fibroblastic cells as well as round cells in clumps (a) Cultures treated with ethanol (1%) and sodium acid succinate (5 to 6 µg/mL) also exhibited fibroblastic morphology with fewer round cells (b). Vitamin E acid succinate-treated cultures [5 µg/mL (c); 6 µg/mL (d)] showed a dramatic change in morphology. x 450 [7].